RODAC – Replicate Organism Detection and Counting, is a technique used in microbiology for environmental monitoring, especially on flat surfaces.
These contact plates used for the surface monitoring of microorganisms in controlled areas such as pharmaceutical, food, and microbiology laboratories.
They contain agar medium (commonly Tryptic Soy Agar – TSA) and are pressed directly onto a surface to collect microorganisms. After incubation, the colonies that grow are counted as CFU (Colony Forming Units) to determine the microbial load on that surface.
Incubation 20-25°C and then 30-35°C, After Incubation colonies that grow on the plate can be counted, indicating the level of microbial cotamination on that surface.
𝐏𝐫𝐨𝐜𝐞𝐝𝐮𝐫𝐞:
- Remove the RODAC plate cover carefully.
- Press the agar surface gently onto the test surface.
- Incubate the plate under suitable conditions.
- Observe and count microbial colonies.
- Record results and compare with acceptable limits.