Author: Sharda Hiremath

SCDA (Soybean Casein Digest Agar), commonly used for general microbiological testing, isnot typically used for water testing because water testing generally requires specialized media that are designed to detect waterborne…

Media WeightTypically, 40 g of SCDA powder is dissolved in 1 liter of distilled water. pHThe pH should be 7.3 ± 0.2 at below 25°C after and before sterilization.pH is…

We use 70% Isopropyl Alcohol (IPA) instead of 100% because it is more effective at killing microorganisms. Pure alcohol evaporates too quickly to penetrate cell walls and denature proteins. 70%…

The UV lamp in a Laminar Airflow (LAF) cabinet typically has a lifespan of around 2000 to 3000 hours of operation, depending on the manufacturer’s specifications. After replacing the UV…

Revalidation of an autoclave typically requires 12 temperature probes strategically distributedthroughout the chamber to ensure uniform sterilization. Key probe placements include: This helps confirm that the autoclave consistently reaches and…

The USP 36-NF 31 <51> states: “The viable microorganisms used in the test must not bemore than five passages removed from the original ATCC culture. The recommendation of five passages…

All cultures must be no more than 5 passages removed from the original stock culture. The working culture should not be subcultured more than 5 times from the original reference…

Supplier QualificationOnly approved suppliers that meet quality standards are chosen. These suppliers mustprovide media that meet lab specifications and are accompanied by a Certificate of Analysis(CoA). Inspection Upon ArrivalUpon receiving…

Temperature and humidity are just two factors that need to be upheld to avoid scrutiny,contamination, comfort for personnel, and maintain a positive safety culture. In the U.S the Food and…