Microbiology

Microbiology

What is the expiry period for the prepared media?

The expiration period for prepared media in microbiology laboratories depends on the type of media, storage conditions, and validation studies conducted.Media expiration dates are primarily based on validation studies that assess how long the media can remain effective under specified storage conditions. Validation involves testing for microbial growth promotion and pH. Validated storage of prepared […]

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Why is treated water sampled before adding sodium thiosulphate to the bottle and sterilization?

Sodium thiosulfate is used to dechlorinate water samples before microbiological testing to ensure the results accurately reflect the water quality at the time of collection.It neutralizes any residual chlorine in the sample and prevents the chlorine from killing bacteria while the sample is being transported to the lab. Sodium thio sulphate is added to the

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Explain deviation.

In the pharmaceutical industry, a deviation is a difference between an observed value and an expected value for a product or process condition. Deviations can also be a departure from a documented standard or procedure. Deviations can occur at any point during a product’s lifecycle, including the testing phase, manufacturing, final product acceptance, and post-market

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What is filler correction, and how many holes are there in the air sampler sieve?

The Feller correction is a mathematical adjustment applied to microbial air sampling to correct for the “overlap” of particles on the sampling plate. When using a microbial air sampler, there is a statistical probability that one or more viablemicroorganisms enter the same hole in the sample head, and impact in the agar forming only one

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What is the temperature of the hot air oven used for drying glassware?

A hot air oven is a laboratory device commonly used for drying, sterilizing, and removing moisture from materials through heated, circulating air. It is widely used in microbiology, pharmaceuticals, and chemical labs, especially where precise temperature control is essential. The temperature of a hot air oven for drying glassware is usually maintained at 160°C for

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What is the size of the Petri plates you are using in the microbiology lab?

Petri plates commonly used in microbiology labs are usually 90 mm in diameter, which is why they are often referred to as 90 mm plates. However, smaller sizes, like 55 mm, or larger sizes, like 150 mm, can also be used depending on the requirements of the experiment. RODAC (Replicate Organism Detection and Counting) plates

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Explain RODAC

RODAC – Replicate Organism Detection and Counting, is a technique used in microbiology for environmental monitoring, especially on flat surfaces. These contact plates used for the surface monitoring of microorganisms in controlled areas such as pharmaceutical, food, and microbiology laboratories. They contain agar medium (commonly Tryptic Soy Agar – TSA) and are pressed directly onto

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