- Sample Preparation
Enrich Sample B in Soybean Casein Digest Medium (SCDM) by incubating at 30-35ยฐC for 24 hours. - Inoculation in MCB
After enrichment, add 1 ml of Sample B to Modified Brilliant Green Bile Broth (MCB).
Incubate this mixture at 42-44ยฐC for 48 hours. - Plating and Incubation
After incubation in MCB, streak the sample onto MacConkey Agar (MCA) plate.
Incubate the MCA plate at 30-35ยฐC for 72 hours. - Result and Interpretation
Observe for brick-red colonies on the MCA plate, which indicate the presence of E. coli (due to lactose fermentation).
